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gad65  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology gad65
    Gad65, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+gad65/GAD-65%2F67+Antibody/pm41729348-80-32-50
    Average 93 stars, based on 60 article reviews
    gad65 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    other:

    Article Title: TRPV1 channels control synaptic plasticity in the developing superior colliculus
    Article Snippet: The same NAPE-PLD and 12LOX antibodies were used as before and coupled, respectively, with anti-GAD65 (1:300, goat antibody; Santa Cruz Biotechnology Inc.).

    Incubation:

    Article Title: Impaired Interneuron Development in a Novel Model of Neonatal Brain Injury
    Article Snippet: Gels were electrotransfered to a 0.2-μm nitrocellulose membrane (Bio-Rad, 1620174). .. Blots were blocked in 5% milk in TBST for 1 h, and then incubated at 4°C overnight with one of the following antibodies: anti-gad65; anti-gad67; anti-SST; anti-gapdh (Santa Cruz Biotechnology, sc377145, sc28376, sc7819, and sc32233, respectively), anti-CLB (Swant Marly, Cb38), and anti-CRT (Millipore Sigma, AB1550). .. Bands were detected with appropriate horseradish peroxide-conjugated secondary antibodies, reacted with chemiluminescent ECL substrate (Bio-Rad, 1705060) and visualized with a Bio-Rad ChemiDoc Imaging system.

    Article Title: S-nitrosylation of GAD65 is implicated in decreased GAD activity and oxygen-induced seizures.
    Article Snippet: Accepted Manuscript Title: S-nitrosylation of GAD65 is implicated in decreased GAD activity and oxygen-induced seizures Authors: Heath G. Gasier, Ivan T. Demchenko, Lynn G. Tatro, Claude A. Piantadosi PII: S0304-3940(17)30473-1 DOI: http://dx.doi.org/doi:10.1016/j.neulet.2017.05.067 Reference: NSL 32874 To appear in: Neuroscience Letters Received date: 20-1-2017 Revised date: 22-5-2017 Accepted date: 30-5-2017 Please cite this article as: Heath G.Gasier, Ivan T.Demchenko, Lynn G.Tatro, Claude A.Piantadosi, S-nitrosylation of GAD65 is implicated in decreased GAD activity and oxygen-induced seizures, Neuroscience Lettershttp://dx.doi.org/10.1016/j.neulet.2017.05.067 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Article Title: Core-shell nanoparticle controlled hATSCs neurogenesis for neuropathic pain therapy.
    Article Snippet: A stem cell-based strategy for tissue engineering in regenerative medicine is crucial to produce and effective therapeutic replacement of injured or damaged tissues.. This type of therapeutic replacement requires interaction with the cells and tissues via the incorporation of a beneficial physical microenvironment and cellular biochemical signals.. Recently, we studied a cell-function modifying factor, coreshell nanoparticles consisting of an SPIO (superparamagnetic iron oxide) core covered with a photonic ZnO shell for human adipose tissue-derived stem cells (hATSCs) that regulate various cellular functions: self-renewal, neurogenesis, and dedifferentiation.

    Staining:

    Article Title: Modulating electrophysiology of motor neural networks via optogenetic stimulation during neurogenesis and synaptogenesis
    Article Snippet: Samples were later stained with primary antibodies at 4 °C overnight, followed by staining of secondary antibodies at room temperature for 2 h. All antibodies were diluted in Antibody Diluent (ThermoFisher Scientific, MA). .. The used antibodies were 1) anti-NeuN antibody for neural populations, (clone A60, Alexa Fluor 555 conjugate), 2) anti-GAD65/67 polyclonal goat antibody (Santa Cruz Biotechnology), 3) anti-vGlut polyclonal guinea pig antibody (Synaptic Systems, Germany), and 4) DAPI as a nuclear stain. .. For synaptophysin clustering quantification between MEBs, the used primary antibody was anti-synaptophysin 1 polyclonal chicken antibody (Synaptic Systems, Germany).

    Immunofluorescence:

    Article Title: The Eph receptor A4 plays a role in demyelination and depression-related behavior
    Article Snippet: Finally, after counterstaining with DAPI, images were captured with an Olympus confocal microscope. .. The following primary antibodies were used in the immunofluorescence assay: anti-EphA4 (Abcam, ab5396, 1:50 or Santa Cruz Biotechnology, sc-365503, 1:50); anti-GFAP (Abcam, ab68428, 1:500); anti-Iba1 (Wako, 019-19741, 1:1000); anti-Vglut1 (Servicebio, GB11821, 1:500); anti-GAD65 and anti-GAD67 (Santa Cruz Biotechnology, sc-365180, 1:100); and anti-MBP (Servicebio, GB12226, 1:800). ..

    Article Title: The Eph receptor A4 plays a role in demyelination and depression-related behavior
    Article Snippet: Finally, after counterstaining with DAPI, images were captured with an Olympus confocal microscope. .. The following primary antibodies were used in the immunofluorescence assay: anti-EphA4 (Abcam, ab5396, 1:50 or Santa Cruz Biotechnology, sc-365503, 1:50); anti-GFAP (Abcam, ab68428, 1:500); anti-Iba1 (Wako, 019-19741, 1:1000); anti-Vglut1 (Servicebio, GB11821, 1:500); anti-GAD65 and anti-GAD67 (Santa Cruz Biotechnology, sc-365180, 1:100); and anti-MBP (Servicebio, GB12226, 1:800). ..

    Western Blot:

    Article Title: Serological Proteome Analysis (SERPA) as a tool for the identification of new candidate autoantigens in type 1 diabetes.
    Article Snippet: Ornella Massa , Massimo Alessio , Lucia Russo , Giovanni Nardo, Valentina Bonetto, Federico Bertuzzi, Alessandra Paladini, Dario Iafusco, Patrizia Patera, Giorgio Federici, Tarcisio Not, Claudio Tiberti, Riccardo Bonfanti, Fabrizio Barbetti k,⁎ Laboratory of Mendelian Diabetes, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy Proteome Biochemistry Unit, San Raffaele Scientific Institute, Milan, Italy Dulbecco Telethon Institute and Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy Endocrinology Unit, Niguarda Hospital, Milan, Italy Department of Pediatrics, Second University of Naples, Naples, Italy Endocrine Unit, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy Bambino Gesù Children's Hospital, IRCCS, Rome Italy Institute for Maternal and Child Health, IRCCS "Burlo Garofalo" and University of Trieste, Trieste, Italy Department of Internal Medicine, University of Rome ‘Sapienza’, Rome, Italy Department of Pediatrics, HS Raffaele Hospital and Scientific Institute, Milan, Italy Department of Experimental Medicine and Surgery, University of Tor Vergata, Rome, Italy



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